| 两种提取蒙古黄芪miRNA方法的比较 |
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| 引用本文： | 吕帝瑾,陈婧,钟扬,南蓬.两种提取蒙古黄芪miRNA方法的比较[J].植物生理学通讯,2014(8):1255-1258. |
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| 作者姓名： | 吕帝瑾 陈婧 钟扬 南蓬 |
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| 作者单位： | 复旦大学生命科学学院生物多样性与生态工程教育部重点实验室,上海200433 |
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| 基金项目： | 国家自然科学基金（81373963和30925004）. |
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| 摘 要： | 通过对两种miRNA提取方法——一步法和多步法进行比较研究，以期获得较高质量的蒙古黄芪不同器官的miRNA。实验结果表明，两种方法均可用于蒙古黄芪miRNA提取，二者存在着不同的优缺点，多步法提取成功率较高但步骤繁琐，相比之下一步法实验条件要求严苛但步骤简单、快捷省时，提取的蒙古黄芪miRNA完整性好，可以满足荧光定量PCR等进一步实验需要。
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| 关 键 词： | miRNA提取 蒙古黄芪 一步法 多步法 |
Compare with Two Protocols for microRNA Extraction from Astragalus membranaceus var. mongholicus |
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| LV Di-Jin,CHEN Jing,ZHONG Yang,NAN Peng.Compare with Two Protocols for microRNA Extraction from Astragalus membranaceus var. mongholicus[J].Plant Physiology Communications,2014(8):1255-1258. |
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| Authors: | LV Di-Jin CHEN Jing ZHONG Yang NAN Peng |
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| Institution: | ( Key Laboratory for Biodiversity Science and Ecological Engineering, Ministry of Education, School of Life Sciences, Fudan University, Shanghai 200433, China) |
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| Abstract: | In order to obtain better quality miRNA in different parts of Astragalus membranaceus var. mongholicus, two miRNA extraction methods （one-step method and multi-step method） were compared. The results showed that these two methods had different advantages and disadvantages, but both of them could be used for miRNA extraction. Multi-step method had higher success rate with cumbersome steps, one-step method needed strict experimental conditions but fast and time-saving, the miRNA isolated with one-step method had high integrity which could be used for quantitative RT-PCR and so on. |
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| Keywords: | miRNA extraction Astragalus membranaceus var mongholicus one-step method multi-step method |
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