The interaction of Ricinus communis hemagglutinin with polysaccharides and low molecular weight carbohydrates |
| |
Authors: | JP Van Wauwe FG Loontiens CK De Bruyne |
| |
Institution: | Laboratorium voor Algemene en Biologische Scheikunde, Rijksuniversiteit van Gent, K. L. Ledeganckstraat 35, B-9000 Gent Belgium |
| |
Abstract: | The hemagglutinin from the castor bean (Ricinus communis) shows a precipitin-like like reaction with a series of branched galactomannas, dependent on their galactose: mannose ratio. Charged and neutral linear galactants fail to co-precipitate with the protein. Hapten inhibition of the turbidimetrically assayed hemagglutinin-Lucerne seed galactomannan system incidates that simple sugars such as D-galactose, D-fucose and L-arabinose bind to the protein. Of the glycosides tested, methyl β-D-galactopyranoside is a better inhibitor than the corresponding α-another. is about 10 tiems less effective than , the best inhibitor tested. Equilibrium dialysis data obatined with the latter ligand are consistent with a protein containing two identical and independent binding sites with an intrinsic association constant equal to 1.65 ? 104 l/mole at 25 °C. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|