Development of chemiluminescence method for determination of 10‐hydroxycamptothecin based on luminol–[Ag(HIO6)2]5− reaction in alkaline solution |
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| Authors: | Hanwen Sun Peiyun Chen Shasha Shi Liqing Li |
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| Institution: | 1. College of Chemistry and Environmental Science, Hebei University, Key Laboratory of Analytical Science and Technology of Hebei Province, Wusi East Road 180, Baoding, 071002, China;2. Department of Chemistry, Taishan University, Taian, 271021, China |
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| Abstract: | A novel chemiluminescence (CL) method was developed for the determination of 10‐hydroxycamptothecin(HCPT) based on the CL reaction between Ag(HIO6)2]5? and luminol in alkaline solution. CL emission of Ag(III) complex–luminol in alkaline medium was very different from that in acidic medium. A possible mechanism of enhanced CL emission was suggested. The enhanced effect of HCPT on CL emission of the Ag(HIO6)2]5?–luminol system was found. The enhanced degree of CL emission was proportional to HCPT concentration. The effect of the reaction conditions on CL emission was examined. Under optimal conditions, the limit of detection was 6.5 × 10?9 g mL?1. The proposed method was applied for the determination of HCPT in real samples with the recoveries of 93.2–109% with the RSD of 1.7–3.3%. Copyright © 2010 John Wiley & Sons, Ltd. |
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| Keywords: | enhanced chemiluminescence Ag(III) complex alkaline medium 10‐hydroxycamptothecin luminol biological fluid |
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