Characterization of a Novel Phosphoinositide-Specific Phospholipase C from Zea mays and its Expression in Escherichia coli |
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| Authors: | Shumei?Zhai,Zhenhua?Sui,Aifang?Yang,Juren?Zhang mailto:jrzhang@sdu.edu.cn" title=" jrzhang@sdu.edu.cn" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author |
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| Affiliation: | (1) School of Life Science, Shandong University, Jinan, 250100, P.R. China |
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| Abstract: | ![]()
A cDNA encoding a phosphoinositide-specific phospholipase C (PI-PLC) has been isolated from Zea mays by screening a cDNA library. The cDNA, designated ZmPLC, encodes a polypeptide of 586 amino acids, containing the catalytic X, Y and C2 domains found in all PI-PLCs from plants. Northern blot analysis showed that the expression of the ZmPLC gene in roots is up-regulated under conditions of high salt, dehydration, cold or low osmotic stress conditions. Recombinant ZmPLC protein was expressed in Esch-erichia coli, purified and used to produce polyclonal antibody, this polyclonal antibody is important for further studies to assess the ultimate function of the ZmPLC gene in plants. |
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| Keywords: | cloning expression phospholipase C polyclonal antibody Zea mays |
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