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Triazole tethered isatin-coumarin based molecular hybrids as novel antitubulin agents: Design,synthesis, biological investigation and docking studies
Authors:Harbinder Singh  Jatinder V. Singh  Manish K. Gupta  Ajit K. Saxena  Sahil Sharma  Kunal Nepali  Preet Mohinder S. Bedi
Affiliation:1. Department of Pharmaceutical Sciences, Guru Nanak Dev University, Amritsar, Punjab 143005, India;2. Lloyd Institute of Management and Technology, Greater Noida, UP, India;3. Indian Institute of Integrative Medicine, Jammu, India
Abstract:
In an attempt to develop potent anti-tubulin agents against most dreadful disease cancer, a library of 28 novel triazole tethered isatin-coumarin hybrids were synthesized by click chemistry approach. Synthesized hybrids were characterized and evaluated against a panel of human cancer cell lines viz. THP-1, COLO-205, HCT-116 and PC-3. Biological assay unveiled that, compounds A-1 to A-6, B-1 to B-4 and C-1 to C-3 displayed significant inhibitory potential against THP-1, COLO-205 and HCT-116 cell lines which were more sensitive towards the designed hybrids. PC-3 among these cell lines was found to be almost resistant. Established SAR revealed marked dependence of the cytotoxic activity on the type of substituent on isatin and the length of carbon-bridge connecting isatin moiety with triazole ring. Unsubstituted isatin and two carbon-bridge were found to be crucial for cytotoxicity. Three most potent hybrids (A-1, A-2 and B-1) were further tested for tubulin polymerization inhibition. Among these three compounds, A-1 found to be endowed with most prominent tubulin polymerization inhibition potential with IC50 value of 1.06 µM which was further confirmed by using confocal microscopy. Possible binding interactions between the most potent hybrid molecule A-1 and tubulin were also divulged by molecular modeling studies.
Keywords:Isatin  Coumarin  Hybrid  Antitumor  Anti tubulin  Docking
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