汾河入黄口夏季微生物群落结构分析

【背景】河流交汇区日益成为流域生态治理的焦点和热点之一。【目的】探明汾河入黄口微生物群落结构及其主要环境影响因子。【方法】应用16S rRNA基因Illumina MiSeq高通量测序技术,分析了汾河入黄口夏季微生物群落结构,并利用典范对应分析(Canonical correspondence analysis,CCA)了解影响微生物群落的主要环境因子。【结果】多样性指数分析表明该区域微生物群落多样性较高。微生物多样性分析发现优势菌门为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)、拟杆菌门(Bacteroidetes)和放线菌门(Actinobacteria);在属分类水平上,相对丰度最高的菌属为芽孢杆菌属(Bacillus),其次为乳球菌属(Lactococcus)和hgcI_clade。Spearman相关性分析及典范对应分析表明环境因子对水体微生物群落结构具有显著影响。【结论】汾河与黄河微生物群落组成具有一定的差异,不同环境因子对不同微生物的影响程度不同,p H和溶解氧(Dissolved oxygen,DO)是汾河入黄口微生物群落结构的主要影响因子。

Microbial community structure of estuary of the Fenhe River into the Yellow River in summer

Background] River confluences have become a focus in ecological management of a river basin. Objective] The microbial community structure and main influencing environmental factors at the estuary of the Fenhe River into the Yellow River were studied. Methods] The microbial community structure at estuary of the Fenhe River into the Yellow River in summer was analyzed by means of 16S rRNA gene-based Illumina Miseq high-throughput sequencing, and the main environmental factors affecting the microbial community were identified by canonical correspondence analysis. Results] The alpha-diversity analysis revealed that the diversity of microbial community was high in this area. Microbial diversity analysis indicated that the dominant bacterial phyla were Proteobacteria, Firmicutes, Bacteroidetes and Actinobacteria; at genus level of taxonomic criteria, the dominant genus was Bacillus, followed by Lactococcus and hgcI_clade. Spearman correlation analysis and canonical correspondence analysis (CCA) showed that environmental factors had significant effects on the microbial community structure of water. Conclusion] The microbial community composition was different between the Fenhe River and the Yellow River, different environmental factors had distinct effects on diverse microorganisms. The microbial communities were mostly affected by pH and dissolved oxygen (DO) at the estuary of the Fenhe River into the Yellow River.