不同诱导子对怀牛膝细胞生长及多糖含量的影响

为提高怀牛膝(Achyranthes bidentata)悬浮培养细胞中牛膝多糖的含量,以酵母提取物、榆黄蘑(Pleurotus citrinopileatus)及水杨酸作为诱导子,分别在同一时期以不同浓度或在不同时期以相同浓度添加至细胞培养物中,收获时测定细胞生长量和牛膝多糖含量。结果显示,在培养12天时添加2.5%(v/v)酵母诱导子,细胞干重最大,可达46.75 g·L–1,多糖含量为5.76 mg·g~(–1);在培养9天时添加5 mg GE·L–1榆黄蘑诱导子,收获时细胞中多糖含量可达6.56 mg·g~(–1),细胞干重达28.3g·L–1;1 mg·g~(–1)水杨酸对牛膝多糖的诱导效果不如以上2种诱导子明显。

Effect of Different Elicitors on Cell Growth and Polysaccharides Content of Achyranthes bidentata

To enhance the polysaccharides content of Achyranthes bidentata suspension cell, yeast extract, Pleurotus citrinopileatus and salicylic acid were added at different concentrations or at different times as the elicitor. With 2.5% (v/v) yeast elicitor added at 12 days, the cell dry weight was the greatest, reaching 46.75 g·L^-1; polysaccharides content was less, 5.76 mg·g^-1. With 5 mgGE·L^-1 fungal elicitor added after culture for 9 days, polysaccharides content could reach 6.56 mg·g^-1; with cell dry weight 28.3 g·L^-1. With 1 mg·g^-1 SA elicitation, the effect was a little inferior to the above two effects.