Transgenic watermelon rootstock resistant to CGMMV (cucumber green mottle mosaic virus) infection |
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Authors: | Sang Mi Park Jung Suk Lee Sung Jegal Bo Young Jeon Min Jung Yoon Sik Park Sang Lyul Han Yoon Sup Shin Nam Han Her Jang Ha Lee Mi Yeon Lee Ki Hyun Ryu Seung Gyun Yang Chee Hark Harn |
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Institution: | (1) Biotechnology Center, Nong Woo Bio, Jeongdan, Ganam, Yeoju, Gyeonggi, 469-884, Korea;(2) Department of Environmental and Life Science, Seoul Womens University, Seoul, 139-774, Korea |
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Abstract: | In watermelon, grafting of seedlings to rootstocks is necessary because watermelon roots are less viable than the rootstock. Moreover, commercially important watermelon varieties require disease-resistant rootstocks to reduce total watermelon yield losses due to infection with viruses such as cucumber green mottle mosaic virus (CGMMV). Therefore, we undertook to develop a CGMMV-resistant watermelon rootstock using a cDNA encoding the CGMMV coat protein gene (CGMMV-CP), and successfully transformed a watermelon rootstock named gongdae. The transformation rate was as low as 0.1–0.3%, depending on the transformation method used (ordinary co-culture vs injection, respectively). However, watermelon transformation was reproducibly and reliably achieved using these two methods. Southern blot analysis confirmed that the CGMMV-CP gene was inserted into different locations in the genome either singly or multiple copies. Resistance testing against CGMMV showed that 10 plants among 140 T1 plants were resistant to CGMMV infection. This is the first report of the development by genetic engineering of watermelons resistant to CGMMV infection. |
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Keywords: | Transformation Cucumber green mottle mosaic virus Coat protein Selection Agrobacterium |
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