Intravascular Immune Surveillance by CXCR6+ NKT Cells Patrolling Liver Sinusoids |
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Authors: | Frederic Geissmann Frederic Geissmann Frederic Geissmann Frederic Geissmann |
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Institution: | 1
Molecular Pathogenesis Program, Skirball Institute of Biomolecular Medicine
New York University School of Medicine, New York, New York
United States of America;2
La Jolla Institute for Allergy and Immunology, San Diego
California
United States of America;3
Millenium Pharmaceuticals, Cambridge
Massachusetts
United States of America;4
Howard Hughes Medical Institute, Skirball Institute of Biomolecular Medicine
New York University School of Medicine, New York, New York
United States of America;University of Minnesota
United States of America |
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Abstract: | We examined the in vivo behavior of liver natural killer T cells (NKT cells) by intravital fluorescence microscopic imaging of mice in which a green fluorescent protein cDNA was used to replace the gene encoding the chemokine receptor CXCR6. NKT cells, which account for most CXCR6+ cells in liver, were found to crawl within hepatic sinusoids at 10–20 μm/min and to stop upon T cell antigen receptor activation. CXCR6-deficient mice exhibited a selective and severe reduction of CD1d-reactive NKT cells in the liver and decreased susceptibility to T-cell-dependent hepatitis. CXCL16, the cell surface ligand for CXCR6, is expressed on sinusoidal endothelial cells, and CXCR6 deficiency resulted in reduced survival, but not in altered speed or pattern of patrolling of NKT cells. Thus, NKT cells patrol liver sinusoids to provide intravascular immune surveillance, and CXCR6 contributes to liver-based immune responses by regulating their abundance. |
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