| 产羧肽酶菌株的筛选及其产酶特性的研究* |
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| 引用本文： | 冯红霞, 陆兆新, 尤华,.产羧肽酶菌株的筛选及其产酶特性的研究*[J].微生物学通报,2003,30(1):38-41. |
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| 作者姓名： | 冯红霞 陆兆新 尤华 |
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| 作者单位： | 南京农业大学食品科技学院,南京,210095 |
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| 基金项目： | 江苏省"10.5"农业科技攻关重点项目 |
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| 摘 要： | 从土壤中分离到28株产蛋白酶菌株,用其粗酶液水解多肽并经氨基酸自动分析仪测定产物中游离氨基酸的含量和种类,从中筛选到一株产羧肽酶的菌株。对该菌株的菌落和菌体形态进行观察,确定其属于曲霉菌属。该菌株在发酵至第48h时,发酵液中羧肽酶活性达到最大。此时发酵液pH值为7.46,菌体已处于衰败期,说明该酶的合成方式为延迟合成型。
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| 关 键 词： | 产羧肽酶菌株 筛选 曲霉菌属 产酶特性 |
| 文章编号： | 0253-2654(2003)01-0038-04 |
| 修稿时间： | 2001年1月21日 |
THE SCREENING OF THE CARBOXYPEPTIDASE PRODUCING STRAIN AND ITS PROPERTIES OF ENZYME PRODUCING |
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| FENG Hong Xia,LU Zhao Xin,YOU Hua.THE SCREENING OF THE CARBOXYPEPTIDASE PRODUCING STRAIN AND ITS PROPERTIES OF ENZYME PRODUCING[J].Microbiology,2003,30(1):38-41. |
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| Authors: | FENG Hong Xia LU Zhao Xin YOU Hua |
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| Abstract: | One carboxypeptidase producing strain was obtained from 28 proteinase producing strains, by analyzing the products of peptides hydrolyzed by the enzyme of the strains According to the characteristics of the morpha and the colonies, the screened strain belongs to aspergillus genera The activity of its carboxypeptidase reached maximum at the 84th hour after fermentation |
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| Keywords: | Carboxypeptidase producing strain Screening Aspergillus genera Properties of enzyme producing |
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