重组毕赤酵母高密度发酵生产内切型纤维素酶的条件优化

为优化内切型纤维素酶高密度发酵工艺条件,在7.5L发酵罐高密度发酵条件下,研究内切型纤维素酶表达量以及毕赤酵母胞外蛋白酶合成水平的影响因素。研究表明:经340mL甘油补料发酵后,在甲醇诱导阶段,pH为5.0,温度为25℃,利用甲醇检测流加控制器控制甲醇体积分数为0.33%～0.35%时,EGI表达量可达421.1IU/mL,比采用固定甲醇流加速率的发酵方法提高了1.49倍。

Optimization of culture conditions for improving the production of endoglucanase in high cell density fermentation of Pichia pastoris

The effect of culture parameters on the expression of endoglucanase and the formation of endogenous protease by high cell-density fermentation in 7.5 L fermenter were optimized.The results show that after 340 mL glycerol fed-batch fermentation,during the process of methanol induction,pH 5.0,temperature 25 ℃,maintaining the residual methanol concentration constantly at range of 0.33% to 0.35% with a methanol sensor,yield of EGI was 421.1 IU/mL,which was 1.49-folds higher in production compared to that with constant methanol feeding rate.