大岩桐花瓣切块离体培养高频率花芽再生

该文报道了大岩桐花瓣切块离体培养再生花现象,花瓣切块再生花有两种方式：一种是仅再生花芽（命名为BF）;另一种是既再生花芽也再生营养芽（命名为BF＋V）。花芽再生的能力与光照、花芽大小及培养基中赤霉素和细胞分裂素浓度紧密相关。当培养基中含有1.0 mg/L GA3时,BA的添加会显著增加总花芽（BF＋BF＋V）的形成率,添加0.5 mg/L BA时,总花芽形成率达100%。在暗中培养时,BF达93.4%。不同大小花芽的花瓣再生花的能力不同,7 mm直径花芽的BF最高,达86.7%。同时,对花芽再生过程中花瓣切块的组织结构形态变化也进行了观察。

High-frequency Floral Bud Regeneration from Petal Segment Cultures of Sinningia speciosa Hiern

The influence of gibberellin and cytokinin on the regeneration of floral bud from petal segments of Sinningia speciosa Hiern in vitro was studied here.Two types of regeneration were regeneration of floral buds only（designated BF）,and regeneration of both floral and vegetative buds（designated BF＋V）.The capacity of floral bud regeneration was influenced by light,the size of the floral bud and exogenous gibberellin（GA3） and cytokinin in the media.In MS basic medium containing 1.0 mg/L GA3,the addition of 6-benzyladenine（BA,0.5 mg/L） significantly increased the frequency of total（BF＋BF＋V） flower bud formation to 100%.The culture was in darkness,the frequency of BF regeneration was up to 93.4%.The highest percentage of BF was 86.7% formed in petal segments from 7 mm floral buds in diameter.Morphological changes in tissue structure of petal segment cultures were observed at several stages（0 to 30 d） under the light microscopy.