光对紫甘蓝花青素合成代谢影响及基因表达模式分析

为了研究光对紫甘蓝花青素合成代谢影响及其调控机制，以“早红”紫甘蓝为试验材料，普通甘蓝“丰园913”（青甘蓝）为对照，对生长1周的幼苗进行遮光处理和光照处理，采用pH差示法测定花青素含量，半定量RT-PCR分析花青素合成途径结构基因表达模式。结果表明：光照处理与遮光处理后，除PAL、UFGT外，紫甘蓝结构基因表达无明显差异，无光条件下，紫甘蓝幼苗仍着色明显，而青甘蓝幼苗完全白化；与青甘蓝相比，紫甘蓝花青素合成途径下游结构基因表达量明显增高，幼苗着色深，揭示紫甘蓝花青素的大量积累与下游结构基因的表达密切相关。

Effect of Light on Anthocyanin Biosynthesis and Metabolism of Brassica oleracea L. and Analysis on Expression Pattern of Anthocyanin Structural Genes

In order to study the influence of light on anthocyanin synthesis and metabolism of purple cabbage and the mechanism of regulation in purple cabbage,"Early Red" purple cabbage was used as the test material,and cabbage "Feng Yuan 913"(green cabbage) was used as the control.Seedlings were planted under light or dark condition for one week,and then the anthocyanin content was determined by using the pH differential spectrum method at pH 1.0 and pH 4.5.Subsequently,semi-quantitative RT-PCR was performed to analyze the expression pattern of structural genes involved in anthocyanin biosynthesis.The results showed that no significant difference was observed in expression level of structural genes involved in anthocyanin biosynthesis in purple cabbage after treated by light or dark except for PAL and UFGT.In addition,purple cabbage seedlings were still colored obviously even grew in the dark,but the young seedlings of green cabbage turned up albefaction completely.The expression levels of downstream structural genes in anthocyanin synthesis pathway were significantly increased in purple cabbage compared with those of green cabbage.At the same time,young seedlings were colored deeply,indicating that mass anthocyanin in purple cabbage was correlated with high expression of these genes.