LOMA: A fast method to generate efficient tagged-random primers despite amplification bias of random PCR on pathogens |
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| Authors: | Wah Heng Lee Christopher W Wong Wan Yee Leong Lance D Miller Wing Kin Sung |
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| Affiliation: | (1) Genome Institute of Singapore, 60 Biopolis Street #02-01, Genome, Singapore;(2) Department of Computer Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore, Singapore |
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| Abstract: | ![]()
Background Pathogen detection using DNA microarrays has the potential to become a fast and comprehensive diagnostics tool. However, since pathogen detection chips currently utilize random primers rather than specific primers for the RT-PCR step, bias inherent in random PCR amplification becomes a serious problem that causes large inaccuracies in hybridization signals. |
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