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Tamm-horsfall glycoprotein: a lectin binding study |
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| Authors: | Beatrice L Griffiths Stefan Karlsson Cecilia P C Soh Anne B St J Dawnay Dallas M Swallow |
| Institution: | (1) MRC Human Biochemical Genetics Unit, University College London, Wolfson House, 4 Stephenson Way, NW1 2HE London, England;(2) Division of Immunochemical Genetics, Clinical Research Centre, Watford Road, HA1 3UJ Harrow, Middlesex, England;(3) Department of Chemical Pathology, St. Bartholomew's Hospital, EC1 London, England;(4) Present address: Department of Health and Human Services, National Heart, Lung and Blood Institute, National Institutes of Health, 20205 Bethesda, Maryland, USA |
| Abstract: | Preparations of Tamm-Horsfall glycoprotein were examined by SDS-polyacrylamide gel electrophoresis and detection using various125I-lectins as well as Coomassie Brilliant Blue. Considerable heterogeneity of electrophoretic pattern was seen. This was not due to a genetic polymorphism. Variation in binding by Soy-bean agglutinin was also seen. This was correlated with the Sda phenotype of the individual. |
| Keywords: | Tamm-Horsfall glycoprotein Sd a antigen 125 I lectins soy-bean agglutinin SDS-gel electrophoresis |
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