DNA banking for plant breeding,biotechnology and biodiversity evaluation |
| |
Authors: | Trevor R Hodkinson Stephen Waldren John A N Parnell Colin T Kelleher Karine Salamin Nicolas Salamin |
| |
Institution: | (1) Department of Botany, School of Natural Sciences, University of Dublin, Trinity College, Dublin, D2, Ireland;(2) Trinity College Botanic Garden, University of Dublin, Trinity College, Dublin, D6, Ireland;(3) Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland;(4) Department of Forest Sciences, University of British Columbia, Vancouver, BC, Canada |
| |
Abstract: | The manipulation of DNA is routine practice in botanical research and has made a huge impact on plant breeding, biotechnology
and biodiversity evaluation. DNA is easy to extract from most plant tissues and can be stored for long periods in DNA banks.
Curation methods are well developed for other botanical resources such as herbaria, seed banks and botanic gardens, but procedures
for the establishment and maintenance of DNA banks have not been well documented. This paper reviews the curation of DNA banks
for the characterisation and utilisation of biodiversity and provides guidelines for DNA bank management. It surveys existing
DNA banks and outlines their operation. It includes a review of plant DNA collection, preservation, isolation, storage, database
management and exchange procedures. We stress that DNA banks require full integration with existing collections such as botanic
gardens, herbaria and seed banks, and information retrieval systems that link such facilities, bioinformatic resources and
other DNA banks. They also require efficient and well-regulated sample exchange procedures. Only with appropriate curation
will maximum utilisation of DNA collections be achieved. |
| |
Keywords: | Databases DNA bank DNA extraction Exchange Management Material transfer agreements |
本文献已被 PubMed SpringerLink 等数据库收录! |
|