MicroRNA-140 Promotes Adipocyte Lineage Commitment of C3H10T1/2 Pluripotent Stem Cells via Targeting Osteopetrosis-associated Transmembrane Protein 1 |
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| Authors: | Yuan Liu Zhi-chun Zhang Shu-wen Qian You-you Zhang Hai-yan Huang Yan Tang Liang Guo Xi Li Qi-Qun Tang |
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| Institution: | From the ‡Key Laboratory of Molecular Medicine, Ministry of Education, and the Department of Biochemistry and Molecular Biology, Fudan University Shanghai Medical College, Shanghai 200032 and ;the §Institute of Stem Cell Research and Regenerative Medicine, Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China |
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| Abstract: | BMP4 has been shown to induce C3H10T1/2 pluripotent stem cells to commit to adipocyte lineage. In addition to several proteins identified, microRNAs also play a critical role in the process. In this study, we identified microRNA-140 (miR-140) as a direct downstream component of the BMP4 signaling pathway during the commitment of C3H10T1/2 cells to adipocyte lineage. Overexpression of miR-140 in C3H10T1/2 cells promoted commitment, whereas knockdown of its expression led to impairment. Additional studies indicated that Ostm1 is a bona fide target of miR-140, which is significantly decreased during commitment, and Ostm1 was also demonstrated to function as an anti-adipogenic factor. |
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| Keywords: | Adipocyte Adipogenesis Mesenchymal Stem Cells Metabolism MicroRNA Molecular Cell Biology |
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