Rapid production of Pediococcus halophilus salt-tolerant cells by a cultivation method employing gradual increases in NaCl concentration using a fermentor with a microfiltration module |
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| Affiliation: | 1. Graduate School of Natural Science and Technology, Japan;2. Department of Chemical Engineering, Faculty of Engineering, Niigata University, Ikarashi, Niigata 950-21, Japan;3. Department of Chemical Engineering, Faculty of Engineering, Nagoya Univeristy, Chikusa-ku, Nagoya 464, Japan;1. Department of Ophthalmology, University of Duesseldorf, Moorenstrasse 5, 40225 Duesseldorf, Germany;2. Institute of Pharmaceutical Technology, Technical University of Braunschweig, Germany;3. Department of Pathology, University of Duesseldorf, Germany;1. Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute», Saint-Petersburg, Russia;2. First Pavlov State Medical University of St. Petersburg, St. Petersburg, Russia;3. CT, SL, AB Sorbonne Universités, UPMC Université, Institut du Cerveau et de la Moelle épinière, ICM, Paris, France;4. Institute of Experimental Medicine, St. Petersburg, Russia;1. Department of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, 6720 Szeged, Eötvös utca 6, Szeged, Hungary;2. Institute of Clinical Microbiology, Faculty of Medicine, University of Szeged, 6725 Szeged, Semmelweis utca 6, Szeged, Hungary;3. Department of Public Health, Faculty of Medicine, University of Szeged, 6720 Szeged, Dóm tér 10, Szeged, Hungary;1. Department of Chemical Engineering, Aristotle University of Thessaloniki (AUTH), P.O. Box: 472, 54124, Thessaloniki, Greece;2. Chemical Process & Energy Resources Institute (CPERI), Centre for Research and Technology Hellas (CERTH), P.O. Box: 60361, 57001, Thermi, Thessaloniki, Greece |
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| Abstract: | ![]()
A new fermentation system employing gradual increases in the NaCl concentration of the culture medium was developed for the rapid production of Pediococcus halophilus NaCl-tolerant cells. A fermentation system with cross-flow filtration using a micro-filtration module allowed the continuous removal of inhibitory metabolites and complete recycling of the cells to the fermentor. By increasing the NaCl concentrations stepwise or linearly in the feeding media, cells of 8.48 or 9.86 g-dry weight per liter were obtained. The productivities of NaCl-tolerant cells per unit time in a cultivation with a stepwise or linear increase in NaCl concentration in the feeding media were 46 or 56-fold as high, repectively, as that in conventional batch cultivation. |
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