甲型流行性感冒病毒自然温度敏感株基因损害定位

采用重组试验和聚丙烯酰胺凝胶电泳(PAGE)技术,把晚期甲3型流感病毒自然ts突变株齐防79-39的ts损害定位在膜蛋白(M)基因上。但互补试验表明,齐防79-39与M基因损害的WSN标准株ts51可以发生互补,这是基因内互补的一个证据。PAGE技术证实,新甲1型流感病毒自然ts株津防77-78的M基因上确有损害。互补试验证明齐防79-39属于一个互补组,而津防77-78与ts51同属于另一个互补组。 本文结果还表明,晚期甲3型齐防79-39的ts损害基因可能是由甲3型野毒株自发突变所产生,而并非通过在自然界与新甲1型重组而获得。

LOCATION OF GENETIC LESION OF NATURALLY OCCURRING TEMPERATURE-SENSITIVE STRAINS OF INFLUENZA A VIRUS

By recombination test and polyacrylamide gel electrophoresis ( PAGE ) analysis, the ts lesion of naturally occurring ts strain of influenza A virus A/Qi/39/79 ( H3N2 ) was located on the matrix protein ( M ) gene.But Qi/39/79 complemented with ts51, a ts strain of WSN virus with M gene lesion.This is considered as evidence for intrasegmental complementation. In this paper, the location of ts lesion of naturally occurring ts strain of HINI influenza virus Tianjin/78/77 on the M gene as previously determined by complementation test was confirmed by recombination and PAGE tests. Thus, Qi/39/79 is a member of one complementation group on the M gene, whereas Tianjin/78/77 and WSN ts51 are members of another complementation group on the same gene.In this paper, PAGE studies showed that there was no evidence of recombination between the genes concerned in ts lesions of H3N2 and new H1N1 virus. Thus, the ts gene of Qi/39/79 may have arisen by spontaneous mutation in nature from the H3N2 wild type virus.