Synthesis and structure determination of [8(-13)C]guanosine 5'-diphosphate |
| |
| Authors: | M Poe J I Germershausen M MacCoss |
| |
| Affiliation: | Department of Biophysics, Merck Sharp & Dohme Research Laboratoires, Rahway, New Jersey 07065. |
| |
| Abstract: | A combined chemical and enzymatic synthesis of [8(-13)C]guanosine 5'-diphosphate (GDP) from H13COOH is described. About 35 mg nucleotide was obtained from 500 mg H13COOH. Analysis of the [8(-13)C]GDP by negative ion fast atom bombardment mass spectrometry and by 13C NMR confirmed that one atom of 13C was incorporated at the 8-position of the guanine ring at 90 +/- 10% enrichment. The chemical shift of the C(8) was 140.2 ppm downfield from internal trimethylsilylpropionate at neutral pH and room temperature, with a spin-spin coupling 1J(13C(8)-H(8] of 217 Hz and a 3J(13C(8)-H(1'] of 3.9 Hz. |
| |
| Keywords: | |
|
|
