| 我国主要地方绵羊品种mtDNA D-loop区PCR-RFLP研究 |
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| 引用本文： | 李祥龙,张增利,巩元芳,刘铮铸,贾青,王立泽. 我国主要地方绵羊品种mtDNA D-loop区PCR-RFLP研究[J]. 遗传, 2006, 28(2): 165-170 |
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| 作者姓名： | 李祥龙 张增利 巩元芳 刘铮铸 贾青 王立泽 |
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| 作者单位： | 1．河北农业大学动物科技学院，保定 071001；2. 河北科技师范学院动物科学系，昌黎 066600 |
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| 摘 要： | 利用5种限制性内切酶（Hinf I，Msp I，Sau3A I，Xsp I，Taq I），采用PCR-RFLP技术研究了我国9个地方绵羊品种以及2个引入品种共计83只绵羊个体线粒体DNA D-loop区的多态性。结果表明，我国主要地方绵羊品种线粒体DNA D-loop区存在两种基本单体型，提示我国主要地方绵羊品种起源于两个母系祖先。线粒体DNA D-loop区多态度为0.042 1%，说明我国地方绵羊品种线粒体DNA多态度较为贫乏。
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| 关 键 词： | D-loop PCR-RFLP 线粒体DNA 地方绵羊品种 |
| 文章编号： | 0253-9772(2006)02-0165-06 |
| 收稿时间： | 2005-02-25 |
| 修稿时间： | 2005-02-25 |
Study on mtDNA D-loop of Chinese Main Indigenous Sheep Breeds Using PCR-RFLP |
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| LI Xiang-Long,ZHANG Zeng-Li,GONG Yuan-Fang,LIU Zheng-Zhu,JIA Qing,WANG Li-Ze. Study on mtDNA D-loop of Chinese Main Indigenous Sheep Breeds Using PCR-RFLP[J]. Hereditas, 2006, 28(2): 165-170 |
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| Authors: | LI Xiang-Long ZHANG Zeng-Li GONG Yuan-Fang LIU Zheng-Zhu JIA Qing WANG Li-Ze |
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| Affiliation: | 1. Department of Animal Science & Technology, Hebei Agriculture University, Baoding,071001，China； 2. Department of Animal Science, Hebei Normal University of Science & Technology, Changli, 066600, China |
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| Abstract: | The polymorphism of mtDNA D-loop of 83 individuals from 9 Chinese indigenous sheep breeds and 2 imported sheep breeds were studied with 5 endonucleases, Hinf I, Msp I, Sau3A I, Xsp I and Taq I, using PCR-RFLP. The results indicated that there existed two basic haplotypes in the region of mtDNA D-loop. It could be inferred that Chinese indigenous sheep breeds originated from two maternal ancestors. The averaged polymorphic degree (Pi value=0.0421%) of mtDNA D-loop showed that the genetic diversity of mtDNA of Chinese indigenous sheep breeds was very poor. |
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| Keywords: | D-loop PCR-RFLP |
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