Effects of polymerase,template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method |
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| Authors: | Jin-Ya Wu Xiao-Tao Jiang Yun-Xia Jiang Su-Ying Lu Fei Zou Hong-Wei Zhou |
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| Affiliation: | 1.Department of Environmental Health, School of Public Health and Tropical Medicine,Southern Medical University,Guangzhou,China |
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| Abstract: | Background The primer and amplicon length have been found to affect PCR based estimates of microbial diversity by pyrosequencing, while other PCR conditions have not been addressed using any deep sequencing method. The present study determined the effects of polymerase, template dilution and PCR cycle number using the Solexa platform. |
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