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In vitro construction of a recombinant adenovirus Ad2:Ad5 |
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| Authors: | Mike Boursnell Vivien Mautner |
| Institution: | MRC Virology Unit, Institute of Virology, Church Street, Glasgow, G11 5JR Scotland |
| Abstract: | A hybrid virus containing the left half of the Ad5 genome and the right half of the Ad2 genome has been constructed by ligating together in vitro the BamHI.-A fragment of Ad5 (map co-ordinates 0–59.5) to the-SawHI-A fragment of Ad2 (map coordinates 59.5–100), and using this DNA to transfect susceptible cells. Viable progeny virus has been obtained which grows as well as the parental virus without any requirement for helper virus, and probably contains a hybrid hexon polypeptide consisting of the major part of the Ad5 hexon with an Ad2 carboxy terminus. |
| Keywords: | Recombinant DNA hybrid hexon polypeptide hexon gene cross-over sites DNA transfection) Ad2 human adenovirus type 2 bp base pairs moi multiplicity of infection pfu plaque-forming units TE 10 mM Tris pH 7 9 1 mM EDTA ts temperaturesensitive |
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