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Localization of the 5S rRNA genes and evidence for diversity in the 5S rDNA region of maize |
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| Authors: | P.N. Mascia I. Rubenstein R.L. Phillips A.S. Wang Lu Zhen Xiang |
| Affiliation: | 1. Department of Genetics and Cell Biology, University of Minnesota, St. Paul, MN 55108 USA;2. Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108 USA |
| Abstract: | The 5S rRNA genes of maize are located in the long arm of chromosome 2 (88% of the distance from the centromere to the end) and organized in a 320-bp repeat. Genomic blots of maize DNA digested with the restriction enzymes BamHI and MspI reveal ladders of bands in multiples of 320 bp. Analysis of 5S rDNA from genomic clones reveals that the ladders are due to both modification and divergence of the 5s rDNA nucleotide sequence. |
| Keywords: | Recombinant DNA Charon 27 vector sequence divergence post-replication modification cloning of plant DNA gene repeats restriction analysis bp base pairs DTT dithiothreitol NOR nucleolus organizer region rDNA coding for ribosomal RNA SDS sodium dodecyl sulfate |
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