Heterologous Expression, Purification, and Characterization of a Highly Active Xylose Reductase from Neurospora crassa |
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| Authors: | Ryan Woodyer Michael Simurdiak Wilfred A. van der Donk Huimin Zhao |
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| Abstract: | A xylose reductase (XR) gene was identified from the Neurospora crassa whole-genome sequence, expressed heterologously in Escherichia coli, and purified as a His6-tagged fusion in high yield. This enzyme is one of the most active XRs thus far characterized and may be used for the in vitro production of xylitol. |
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