Sialic acid-containing lipopolysaccharides in purple nonsulfur bacteria |
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| Authors: | J H Krauss G Reuter R Schauer J Weckesser H Mayer |
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| Institution: | (1) Max-Planck-Institut für Immunbiologie, Stübeweg 51, D-7800 Freiburg, Federal Republic of Germany;(2) Biochemisches Institut, Christian-Albrechts-Universität, Olshausenstrasse 40, D-2300 Kiel, Federal Republic of Germany;(3) Institut für Biologie II, Mikrobiologie, der Albert-Ludwigs-Universität, Schänzlestrasse 1, D-7800 Freiburg, Federal Republic of Germany |
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| Abstract: | Lipopolysaccharides (LPS) from a number of purple nonsulfur bacteria and of phylogenetically related species were analyzed for the presence of sialic acid by gas chromatography/mass spectrometry. Species and strains of the genera Rhodobacter, Rhodopseudomonas, Rhodomicrobium, Rhodospirillum, Rhodocyclus and Rhodopila were investigated, sialic acid, however, was found only in the genus Rhodobacter. It occurs in strains of Rhodobacter capsulatus, R. sphaeroides, R. sulfidophilus and R. veldkampii. All these species belong to the  -3 subgroup of purple bacteria as defined by 16S rRNA catalogues. Approximately equimolar ratios of sialic acid and of 2-keto-3-deoxy-octonate (KDO) were found in isolated LPSs. Sodium deoxycholate gel electrophoresis of these LPS-samples also suggested a location of sialic acid in the LPS  core region. Sialic acid was present only in those LPSs, which exhibited a complete core region.Abbreviations
LPS
Lipopolysaccharide
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Neu5Ac
N-acetylneuraminic acid
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KDO
2-Keto-3-deoxy-octonate
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Neuß2Me
neuraminic acid- 2-methyl glycoside
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DOC
sodium deoxycholate
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PAGE
polyacrylamide gel electrophoresis
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HPLC
high performance liquid chromatography
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TLC
thin layer chromatography |
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| Keywords: | Lipopolysaccharide Sialic acids Core region Purple nonsulfur bacteria N-Acetylneuraminic acid Rhodobacter |
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