The genetic structure of populations of the vulnerable aquatic macrophyte <Emphasis Type="Italic">Ranunculus nipponicus</Emphasis> (Ranunculaceae) |
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Authors: | Keiichi Koga Yasuro Kadono Hiroaki Setoguchi |
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Institution: | (1) Department of Biology, Graduate School of Human and Environmental Studies, Kyoto University, Kyoto 606-8501, Japan;(2) Department of Biology, Faculty of Science, Kobe University, Kobe 657-8501, Japan |
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Abstract: | Ranunculus nipponicus (Makino) Nakai is a vulnerable aquatic macrophyte in the Kinki district, which is the southernmost distribution of this species
in Japan. The genetic diversity and structure within and among eleven extant populations were assessed using the inter-simple
sequence repeats (ISSR) polymerase chain reaction in association with combinations of propagation pattern (clonal and/or seeds)
and genotypic geographical structure. In total, 53 bands were amplified, of which 18 (34%) were polymorphic. Analysis of the
ISSR bands identified 46 genotypes among 81 individuals from one stream population and 72 distinct genotypes among 147 individuals
in the Kinki district. An unweighted pair group method with arithmetic mean (UPGMA) dendrogram showed some unity among upstream
and downstream subpopulations within one stream and eleven populations. The Shannon index of genetic diversity was 0.109 for
one stream population and 0.313 for total genetic diversity, suggesting relatively high genetic diversity. Analysis of molecular
variance (AMOVA) revealed that 84.1% of the total genetic diversity occurred among populations and the remaining diversity
(15.9%) occurred within populations. Significant genetic differentiation occurred among populations in the Kinki district.
These results suggest that conservation of each population is important for maintaining genetic diversity of R. nipponicus in this district.
An erratum to this article can be found at |
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Keywords: | Aquatic plant Conservation Genetic diversity ISSR Ranunculus nipponicus |
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