3-Hydroxypropyl amide formation from 1-aminocyclopropane-1-carboxylic acid by a cell-free ethylene-forming system from olive leaves |
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Authors: | Blanca Vioque Juan Carlos Fernández-Maculet |
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Institution: | Dpto de Fisiologia y Technología Post-Recolección, Inst. de la Grasa y sus Derivados, Avda Padre García Tejero 4, E-41012 Sevilla, Spain |
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Abstract: | The low ethylene yield in a cell-free ethylene-forming system from olive tree leaves ( Olea europaea L. cv. Picual) was investigated. During the incubation, 1-aminocyclopropane-1-carboxylic acid (ACC) was extensively transformed into 3-hydroxypropyl amide (HPA). Enzyme extract, Mn2+ and oxygen are responsible for this reaction. Horseradish peroxidase (EC 1.11.1.7) can substitute for the enzyme extract in this reaction. HPA formation could be one reason for the poor in vitro conversion efficiency of ACC to ethylene. |
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Keywords: | 1-Aminocyclopropane-1-carboxylic acid ethylene 3-hydroxypropyl amide Olea europaea olive tree peroxidase |
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