狂犬病毒抗体ELISA检测试剂盒的改进研究

为了提高狂犬病毒抗体检测的灵敏度和特异性，采用狂犬病毒单克隆抗体包被酶标板，再分别加入重组的狂犬病毒糖蛋白或细胞培养抗原做固相层的方法(抗体捕捉法)，用传统的间接ELISA法做对照，按常规方法检测抗狂犬病毒抗体。结果显示，抗体捕捉法的非特异性反应低于间接法，而灵敏度达到0．51U水平，高于间接法。在800份临床标本检测中，检出率明显高于间接法。用15份阳性血清作小鼠中和试验，并和抗体捕捉ELISA法比较具有高度的一致性。试验结果充分表明，该方法优于传统的ELISA间接法。因此可作为临床注射狂犬疫苗后检测血清中狂犬病毒抗体的常规方法。

Improvement of ELISA Kit for detection of antibody against rabies virus

In order to improve the sensitivity and the specificity for detection of antibody against rabies virus,the 96 well microtitre plates were coated with monoclonal rabbies antibodies,and then the recombinant glycoprotein or cultured antigen of rabies virus were loaded as solid phrase to detect the rabies antibodies (antibody capture assay,ACA).The indirecct-ELISA(traditional assay)method was used as comparison.The results showed that the ACA method is better specificity and higher sensitivity than indirect-ELISA method,0.5IUof antibodies could be detectde.The detection rate 800 clinical serum specimens was higher than indirect-ELISA method apparently.In comparative test of ACA method and mouse neutralization assay by using 15 specimens of positive sera,the results demonstrated that the two methods have high agreement.All results indicated that the ACA method were better than indirect-ELISA method,and could be used to detect the rabies antibody in clinic.