| DNA条形码在生态学研究中的应用与展望 |
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| 引用本文： | 罗亚皇,刘杰,高连明,李德铢.DNA条形码在生态学研究中的应用与展望[J].云南植物研究,2013(6):761-768. |
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| 作者姓名： | 罗亚皇 刘杰 高连明 李德铢 |
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| 作者单位： | [1]中国科学院昆明植物研究所生物多样性与生物地理学重点实验室,云南昆明650201 [2]中国科学院西南野生生物种质资源库,云南昆明650201 [3]中国科学院大学,北京100049 [4]云南大学生命科学学院,云南昆明650091 |
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| 基金项目： | 科技部重大研究计划项目（2014CB954100）;科技基础性工作专项项目（2012FY110800,2013FY112600）;中国科学院大科学装置项目（2009-LSFGBOWS-01） |
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| 摘 要： | DNA条形码是利用标准的DNA片段对物种进行快速鉴定的技术，已在生物学各相关领域得到广泛应用。随着DNA条形码技术的不断发展和完善，已成功应用于生态学领域的相关研究中。本文综述了DNA条形码在物种快速鉴定和隐存种发现、群落系统发育重建和生态取证、群落内物种间相互关系研究等方面的应用，并介绍了DNAmetabarcoding技术和环境DNA条形码在生物多样性和生态学研究领域中的应用。最后，结合新的测序技术和未来大科学装置的发展，在相关数据库逐渐完善，新分析方法和计算模型不断开发使用的情景下，对DNA条形码在生态学相关领域的应用前景进行了展望。
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| 关 键 词： | 生物多样性 群落系统发育 DNA metabarcoding 环境DNA条形码 第二代测序技术 |
Applications and Advances of DNA Barcoding in Ecological Studies |
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| LUO Ya-Huang,LIU Jie,GAO Lian-Ming,LI De-Zhu.Applications and Advances of DNA Barcoding in Ecological Studies[J].Acta Botanica Yunnanica,2013(6):761-768. |
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| Authors: | LUO Ya-Huang LIU Jie GAO Lian-Ming LI De-Zhu |
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| Institution: | 1 Key Laboratory of Biodiversity and Biogeography, Kunming lnstitute of Botany, Chinese Academy of Sciences, Kunming 650201, China; 2 Germplasm Bank of Wild Species in Southwest China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China ; 3 University of Chinese Academy of Sciences, Beijing 100049, China ; 4 School of Life Sciences, Yuanan University, Kunming 650091, China) |
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| Abstract: | DNA barcoding is a technique used to identify species, which is now widely employed in different biologi- cal disciplines. With the development and modification of DNA barcoding, it has become a useful tool for ecological studies. Here, we review the uses of DNA barcoding for quick species identification and/or new and cryptic species discovery, in community phylogenetic reconstruction and ecological forensics, and in interaction networks among species within a community. The techniques of DNA metabarcoding and environmental DNA metabarcoding used in ecological research such as diet analysis and biodiversity assessment are also examined. Finally, we discuss the fu- ture prospects of DNA barcoding in ecological research following the development of next-generation sequencing tech- nologies, improvement of Large-scale Scientific Facilities, and use of new models and software for data analysis. |
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| Keywords: | Community phylogeny Environmental DNA barcoding DNA metabarcoding Biodiversity Next gener- ation sequencing |
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