Simultaneous Determination of Oxidized and Reduced Forms of Plastoquinone A in Spinach Chloroplasts by High-Performance Liquid Chromatography with an Electrochemical Detector |
| |
Authors: | Isogai Yasuhiro; Nishimura Mitsuo; Okayama Shigeki |
| |
Institution: | 1Department of Biology, Faculty of Science, Kyushu University Fukuoka 812, Japan
2Biological Laboratory, College of General Education, Kyushu University Fukuoka 810, Japan |
| |
Abstract: | A method for determination of the redox level of plastoquinoneA in spinach chloroplasts is described. Plastoquinone A andits reduced form plastoquinol A were extracted from chloroplastson a sample-preparation cartridge (SEP-PAK C18 Cartridge, WatersAssoc. Inc.) with a mixture of ethanol and diethyl ether ( 1: 1, vv). Extracts were separated by reversed-phase high-performanceliquid chromatography and examined with an electrochemical detectorequipped with dual electrodes. Plastoquinone A was determinedby its reductive current on one electrode, and plastoquinolA by its oxidative current on the other electrode. This method was applied to the determination of the redox potentialof plastoquinone A in chloroplasts. The midpoint potential atpH 7.8 of plastoquinone A was +20 mV with an n number of 2. (Received March 30, 1987; Accepted August 3, 1987) |
| |
Keywords: | |
本文献已被 Oxford 等数据库收录! |
|