A rapid non-destructive DNA extraction method for insects and other arthropods |
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| Authors: | Mark A Castalanelli Dustin L Severtson Cameron J Brumley Andras Szito Robert G Foottit Mike Grimm Kylie Munyard David M Groth |
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| Institution: | 1. Cooperative Research Centre for National Plant Biosecurity, Bruce, ACT, 3 Baron-Hay Crt, South Perth, 6151, Australia;2. Curtin Health Innovation Research Institute, Western Australian Biomedical Research Institute, Curtin University of Technology, GPO Box U1987, Perth WA, 6845, Australia;3. Division of Biosecurity and Research, Department of Agriculture and Food Western Australia, WA, Baron-Hay Crt, South Perth, 6151, Australia;4. National Environmental Health Program—Invertebrate Biodiversity, Agriculture and Agri-Food/Agriculture et Agroalimentaire Canada, Research Centre, K. W. Neatby Building, 960 Carling Avenue, Ottawa, Ontario, Canada K1A 0C6 |
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| Abstract: | Preparation of arthropods for morphological identification often damages or destroys DNA within the specimen. Conversely, DNA extraction methods often destroy the external physical characteristics essential for morphological identification. We have developed a rapid, simple and non-destructive DNA extraction technique for arthropod specimens. This technique was tested on four arthropod orders, using specimens that were fresh, preserved by air drying, stored in ethanol, or collected with sticky or propylene glycol traps. The technique could be completed in 20 min for Coleoptera, Diptera and Hemiptera, and 2 min for the subclass Acarina, without significant distortion, discolouration, or other damage to the specimens. |
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