Acquired immune response to defined Plasmodium vivax antigens in individuals residing in northern India |
| |
| Authors: | Ratanesh K Seth Ajaz A Bhat Donthamsetty N Rao Sukla Biswas |
| |
| Institution: | 1. South Carolina Statewide Cancer Prevention & Control Program, University of South Carolina, Columbia, SC, United States;2. Department of Epidemiology and Biostatistics, Arnold School of Public Health, University of South Carolina, Columbia, SC, United States;3. Dorn Department of Veterans Affairs Medical Center, Columbia, SC, United States;4. Environmental Health & Disease Laboratory, Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, SC, United States;1. Water and Health Laboratory, Cyprus International Institute for Environmental and Public Health, Cyprus University of Technology, Limassol 3041, Cyprus;2. Environmental Health Sciences Dept., University of South Carolina, United States;1. Environmental Health and Disease Laboratory, Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, SC, USA;2. Department of Cancer Biology and Pharmacology, University of Illinois College of Medicine, Peoria, IL, USA;3. Department of Health Services Policy and Management, Arnold School of Public Health, University of South Carolina, Columbia, SC, USA;4. Department of Environmental Health, Boston University School of Public Health, Boston University, Boston, MA, USA;5. Department of Pathology Microbiology and Immunology, USC School of Medicine, University of South Carolina, Columbia, SC, USA;1. Environmental Health and Disease Laboratory, Department of Environmental Health Sciences, Arnold School of Public Health, University of South Carolina, Columbia, SC 29208, USA;2. Free Radical Metabolism Group, Laboratory of Toxicology and Pharmacology, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA;3. Division of Gastroenterology, Duke University, Durham, NC 27707, USA;4. Dept. of Pharmaceutical Sciences, University of Connecticut, Storrs, CT 06269-3092, USA |
| |
| Abstract: | In this investigation, we evaluated the naturally acquired immune response to Plasmodium vivax stage-specific antigens in individuals of different age groups belonging to malaria endemic areas of northern India.Four synthetic peptides containing both B- and T-cell epitopes from P. vivax circumsporozoite protein, merozoite surface protein-1, apical membrane antigen-1 and gametocyte surface antigen-1 were used to determine both humoral and cellular immune responses.Immunity, in terms of antibody response and T-cell proliferation against these stage-specific peptides, has been observed in the study subjects. The results demonstrated age-dependent antibody response in this population. Forty two patients were diagnosed with P. vivax. There was a significant association (P = 0.013) between number of antibody responders and recognition of stage-specific epitopes by antibodies. The antibody response to B-epitopes of P. vivax CSP, MSP1, AMA1 and GAM1 was associated with age; adults responded more frequently to these antigens than did younger children. In this population, 66% (201/304) cases showed seropositivity to all peptides and 13% (41/304) showed negative response. Peripheral blood mononuclear cells of more than 75% of individuals proliferated in response to stimulation by all four epitopes.In conclusion, the results demonstrated immunogenicity of the epitopes to P. vivax in population of this endemic zone. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
