Evaluation of the antigenicity of universal epitopes from PvDBPII in individuals exposed to Plasmodium vivax malaria |
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| Authors: | Paola Martinez Carolina Lopez Carolina Saravia Magnolia Vanegas Manuel A Patarroyo |
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| Institution: | 1. Fundación Instituto de Inmunología de Colombia (FIDIC), Carrera 50 No. 26-20, Bogotá, Colombia;2. Universidad del Rosario, Calle 63D No. 24-31, Bogotá, Colombia;3. Universidad Nacional de Colombia, Carrera 45 No 26-85, Bogotá, Colombia;1. Hematology and Clinical Immunology Section, Department of Clinical and Experimental Medicine, University of Perugia, Italy;;2. Hematology Section, Department of Life, Health and Environmental Sciences, University of L''Aquila, Italy;;3. Weizmann Institute of Science, Immunology Department, Rehovot, Israel; and;4. Hematology and Bone Marrow Transplantation Unit, Department of Clinical and Experimental Medicine, University of Parma, Italy;1. Department of Vaccine Development, La Jolla Institute for Allergy and Immunology, 9820 Athena Circle, La Jolla, CA 92037, USA;2. Bioinformatics Core Facility, La Jolla Institute for Allergy and Immunology, 9820 Athena Circle, La Jolla, CA 92037, USA;1. GlaxoSmithKline Vaccines, Rixensart, Belgium;2. Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand;3. Malaria Vaccine Branch, Walter Reed Army Institute of Research, Silver Spring, MD, USA;1. Immunology Programme, Life Sciences Institute, National University of Singapore, Singapore;2. Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore;3. Infectious Disease Interdisciplinary Research Group, Singapore-MIT Alliance for Research and Technology, National University of Singapore, Singapore;4. Defense Medical and Environmental Research Institute, Defense Science Organization National Laboratories, Singapore;5. Koch Institute for Integrative Cancer Research and Department of Biology, Massachusetts Institute of Technology, Cambridge, MA |
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| Abstract: | The Duffy-binding protein (PvDBP) mediates invasion of reticulocytes by the malaria parasite Plasmodium vivax. PvDBP has been recognized as a good vaccine candidate due to its ability to induce antibody responses capable of inhibiting target cell invasion after natural infections. For the development of subunit-based vaccines, it is important to identify universal epitopes that could be presented by different HLA-DR alleles to induce effective cellular and humoral immune responses. In this study, the antigenicity of universal epitopes from PvDBPII was evaluated by stimulating peripheral blood mononuclear cells (PBMCs) isolated from individuals with different degrees of P. vivax malaria exposure and distinct HLA-DR alleles. Peptides 1635 and 1638 induced lymphoproliferation and stimulated the production of IL-6 and IFN-γ. The results suggest that conserved peptides binding with high activity to red blood cells and with known affinity to HLA-DR proteins could be good components for a P. vivax vaccine. |
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