Praziquantel has no direct effect on (Na++K+)-ATPases and (Ca2+-Mg2+)ATPases of Schistosoma mansoni |
| |
| Institution: | 2. Department of Biomedicine, University of Basel, Basel, Switzerland;3. Doctorate School in Clinical and Experimental Medicine, University of Modena and Reggio Emilia, Modena, Italy;4. TYDOCK PHARMA srl, Modena, Italy;5. Institute for Molecular and Cell Biology, Porto, Portugal;6. Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal;1. Department of Parasitology, Leiden University Medical Center, Leiden, The Netherlands;2. Bernhard-Nocht-Institut für Tropenmedizin, Hamburg, Germany;3. German Center for Infection Research, Germany;4. Centre de Recherches Médicales de Lambaréné, Lambaréné, Gabon;5. Eberhard Karls Universität Tübingen, Tübingen, Germany;6. Centre d''Infectiologie Charles Mérieux, Antananarivo, Madagascar;7. Department of Cell and Chemical Biology, Leiden University Medical Center, Leiden, The Netherlands;8. Université de Fianarantsoa, Fianarantsoa, Madagascar;9. Université d''Antananarivo, Antananarivo, Madagascar;10. ISGlobal, Hospital Clínic, Universitat de Barcelona, Barcelona, Spain;11. Health Economics Group, Department of Infectious Disease Epidemiology, School of Public Health, Imperial College London, London, UK |
| |
| Abstract: | Therapeutic concentrations of praziquantel produce a rapid and intense contraction of the human flatworm Schistosoma mansoni. As an action on ATPases responsible for calcium homeostasis arises as a possible explanation for the molecular mechanism of this effect, we tested here the effect of praziquantel on different preparations from male adult worms that were previously characterized for their content in (Na++K+)-ATPase and (Ca2+-Mg2+)ATPase activities from different origins. Concentrations as high as 100 μM praziquantel did not inhibit (Na++K+)-ATPase from tegument and carcass nor (Ca2+-Mg 2+)ATPase from heterogeneous (P1) and microsomal (P4) fractions. As 100 μM praziquantel was also without effect on calcium permeability of microsomal vesicles actively loaded with 45Ca2+, the present results discard three hypotheses recently raised for the mechanism of praziquantel-induced contraction of S. mansoni. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
