Interaction of aflatoxin B1 with electron-donating organic molecules,including aromatic amino acids |
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Authors: | Icksam Noh Fun Sun Chu |
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Institution: | 1. Food Research Institute University of Wisconsin, Madison, Wisconsin 53706 USA;2. Department of Food Science, University of Wisconsin, Madison, Wisconsin 53706 USA |
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Abstract: | The interaction of the carcinogenic mycotoxin, aflatoxin B1, with some electrondonating organic compounds including aromatic hydrocarbons, dimethylaniline, and aromatic amino acids, was studied. Spectrophotometric analysis of aflatoxin B1 revealed that hypochromicity in the absorption around 360 nm and hyperchromicity around 385 nm were induced by dimethylaniline, hexamethylbenzene, tryptophan, and imidazole. A similar shifting of aflatoxin B1 absorption was observed in benzene, toluene, and xylene in the presence of ZnCl2. The interaction of aflatoxin B1 with polystyrene was observed in a biphasic system. The association constants of aflatoxin B1: DMA4 (1:1) and of aflatoxin B1: tryptophan (1:1) were found to be 0.64 and 22.6 liters per mole, respectively. The results suggest that charge-transfer interaction occurs between aflatoxin B1 and these π-electron donors. Since the spectral changes on aflatoxin B1 absorption induced by these π-electron donors are similar to those induced by nucleic acids and proteins, it is postulated that charge-transfer interaction also occurs between aflatoxin B1 and these macromolecules. The role of such interaction in the biological activity of aflatoxin B1 is discussed. |
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