Electrofusion of Neurospora crassa slime cells |
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| Authors: | Magdalena Fikus,El&# bieta Grzesiuk,Piotr Marsza&# ek,Stefan Ró &# ycki,Jerzy Zieli&# ski |
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| Affiliation: | Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 02-532 Warszawa, Rakowiecka 36, Poland;Electrotechnical Research Institute, Department of Fundamental Research in Electrotechnics, 04-703 Warszawa, Pożaryskiego 28, Poland |
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| Abstract: | Abstract Optimal conditions were established for cell growth, dielectrophoresis and electrofusion of Neurospora crassa slime mutants. It was concluded that these methods could be applied to genetic manipulations (i.e. transformation and construction of diploid slime variants) of N. crassa protoplasts. |
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| Keywords: | Dielectrophoresis fusion fungal protoplasts |
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