Effect of modified glucose catabolism on xanthan production in <Emphasis Type="Italic">Xanthomonas oryzae</Emphasis> pv. <Emphasis Type="Italic">oryzae</Emphasis> |
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Authors: | Se-Gul Jang Byoung-Moo Lee Jae-Yong Cho |
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Institution: | (1) Department of Pharmaceutical Engineering, College of Health Science, Sangji University, Sangjidae-gil 83, Woosan-dong, Wonju-si, Gangwon-do, 220-702, Korea;(2) Microbial Genetics Division, National Institute of Agricultural Biotechnology, 225 Seodun-dong, Suwon, 441-707, Korea;(3) labGene Inc., Sangji University, Sangjidae-gil 83, Woosan-dong, Wonju-si, Gangwon-do, 220-702, Korea; |
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Abstract: | In this study, the glucose 6-phosphate dehydrogenase gene (XOO2314) was inactivated in order to modulate the intracellular
glucose 6-phosphate, and its effects on xanthan production in a wild-type strain of Xanthomonas oryzae were evaluated. The intracellular glucose 6-phosphate was increased from 17.6 to 99.4 μmol g−1 (dry cell weight) in the gene-disrupted mutant strain. The concomitant increase in the glucose 6-phosphate was accompanied
by an increase in xanthan production of up to 2.23 g l−1 (culture medium). However, in defined medium supplemented with 0.4% glucose, the growth rate of the mutant strain was reduced
to 52.9% of the wild-type level. Subsequently, when a family B ATP-dependent phosphofructokinase from Escherichia coli was overexpressed in the mutant strain, the growth rate was increased to 142.9%, whereas the yields of xanthan per mole of
glucose remained approximately the same. |
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