Composition ofNeurospora crassa vacuolar membranes and comparison to endoplasmic reticulum,plasma membranes,and mitochondrial membranes |
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| Affiliation: | 1. College of Food Science and Technology & MOE Key Laboratory of Environment Correlative Dietology, Huazhong Agricultural University, Wuhan 430070, China;2. College of Food and Biotechnology, Wuhan Institute of Design and Science, Wuhan 430205, China;3. Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology and Business University (BTBU), Beijing 100048, China;1. Institute for Micrsensors, -actuators and –systems (IMSAS), University of Bremen, Otto-Hahn-Allee NW1, 28359 Bremen, Germany;2. Microsystems Center Bremen (MCB), Germany;3. microFAB Service GmbH, Bremen, Germany;1. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Beijing, China;2. The Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou, China;3. Fungal Genomics Group, College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing, China;4. Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, TN, USA |
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| Abstract: | We have determined the carbohydrate and lipid contents of vacuolar membranes fromNeurospora crassa, and have compared them to mitochondrial membranes, endoplasmic reticulum, and plasma membranes. These four membrane fractions were clearly distinct from each other in polypeptide composition, as judged by polyacrylamide gel electrophoresis. The vacuolar membranes proved unusual in two respects: the contained very high amounts of carbohydrate and were the only membranes with significant levels of phosphatidylserine. As in other eucaryotic cells, the mitochondrial membranes were unique in having high amounts of cardiolipin but virtually no sterol. Although the endoplasmic reticulum and plasma membranes were qualitatively similar to each other, the plasma membranes could be distinguished by a higher carbohydrate content, whereas the endoplasmic reticulum had a characteristically high ratio of phosphatidylcholine to phosphatidylethanolamine. The fatty acid compositions of all four membranes were similar, except that mitochondrial membranes contained about half as much saturated fatty acids as the other three fractions. |
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