Effects of long-term cryopreservation on peripheral blood progenitor cells |
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| Authors: | Gregory S Vosganian Jill Waalen Kevin Kim Sejal Jhatakia Ethan Schram Tracey Lee Dan Riddell James R Mason |
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| Institution: | 1. Scripps Clinic and Green Hospital, Division of Hematology/Oncology, La Jolla, California, USA;2. The Scripps Research Institute, La Jolla, California, USA;3. Kootenai Cancer Center, Coeur d’Alene, Idaho, USA;4. Kaiser Permanente Medical Center, Department of Hematology/Oncology, San Jose, California, USA;5. Martin-O''Neil Cancer Center at St Helena Hospital, St Helena, California, USA;6. Scripps Green Hospital-Stem Cell Processing Center, La Jolla, California, USA;7. Blood and Marrow Transplantation Program, Scripps Clinic and Green Hospital, La Jolla, California, USA;1. Department of Experimental Medicine, McGill University, Montreal, Canada;2. Lady Davis Institute for Medical Research, Montreal, Canada;3. Department of Pathology and Cell Biology, Faculty of Medicine, Université de Montréal, Montreal, Canada;4. Department of Pediatrics and Department of Hematology and Medical Oncology, Emory University & Emory University Winship Cancer Institute, Atlanta, Georgia, USA;1. CHU Sainte-Justine Research Center, Center of Cancerology Charles-Bruneau, Montreal, Quebec, Canada;2. Department of Microbiology and Immunology, University of Montreal, Montreal, Quebec, Canada;3. Department of Paediatrics, University of Montreal, Montreal, Quebec, Canada;1. Regenerative Medicine Institute, NUI Galway, Galway, Ireland;2. Department of Neurology, Mayo Clinic, Rochester, Minnesota, USA;3. Discipline of Anatomy, NUI Galway, Galway, Ireland;4. Department of Anatomy, University College Cork, Cork, Ireland;5. School of Biomedical Sciences, Charles Sturt University, Wagga Wagga, New South Wales, Australia |
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| Abstract: | Background aimsThe long-term stability of cryopreserved peripheral blood progenitor cells is an important issue for patients experiencing disease relapse. However, there is no consensus on how to evaluate the long-term effects of cryopreservation. We describe the effect of cryopreservation on viability and progenitor colony activity from 87 individual samples processed at the Scripps Green Hospital Stem Cell Processing Center (La Jolla, CA, USA).MethodsWe randomly selected 87 peripheral blood hematopoietic stem cell (PBHSC) samples from 60 patients and evaluated the effect of cryopreservation on sample viability and red and white cell colony activity after < 24 h and 7, 10 and 15 years of cryopreservation. Viability was assayed via trypan blue dye exclusion and activity was measured following 14 days of culture.ResultsAn age at collection older than 50 years may result in suboptimal activity and viability following long-term cryopreservation, while gender and disease status had no effect. Cryopreservation did not significantly affect white or red cell activity following 10 years of cryopreservation. However, for samples stored longer than 10 years, viability and activity significantly decreased. We noted a positive association between higher pre-cryopreservation %CD34 count and colony activity.ConclusionsCryopreservation of peripheral blood progenitor cells for up to 10 years results in no loss of clonogenic capacity, as determined by culture activity, although longer durations of storage may affect activity. Until validated methods are developed, cryopreserved grafts should be evaluated based on pre-freeze CD34+ cell counts as assayed by flow cytometry, and post-thaw sample evaluation should be reserved for patients identified as poor mobilizers. |
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