月光花素对彩叶芋愈伤组织细胞生长和分化的影响

在含有NAA0.2mg/L,2.4-D 0.2mg/L的MS基础培养基中添加月光花素培养彩叶芋愈伤组织,结果显示,低浓度的月光花素能促进愈伤组织生长,高浓度则有明显的抑制作用,最适浓度为1.0~2.0mg/L;培养两个月后,添加适宜浓度的月光花素(0.2~2.0mg/L)的培养基能促进体细胞分化和植株再生,而高浓度(10.0mg/L)处理和对照均未能分化出绿苗。表明适宜浓度的月光花素在合适的生长素剂量的协同作用下,对植物愈伤组织细胞的生长和分化有较强的调节作用。

Effects of calonyctin on growth and differentiation of Caladiumbicolor callus

Caladium bicolor callus was cultured on the basic MS media containing calonyctin supplemented with 0.2mg/L NAA and 0.2mg/L 2,4-D. The results showed that 0.2~2.0mg/L calonyctin promoted the growth and differentiation of Caladium bicolor callus. After culturing for 2 months, the callus differentiated into plantlets on the medium 0.2~2.0mg/L calonyctin while both control and the callus treated with high concentration of calonyctin (10.0mg/L) did not regenerated plantlets. The results suggested that calonyctin with proper growth regulators has regulatory effects on the growth and differentiation of plant callus.