Regulated production of recombinant echistatin by yeast |
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| Authors: | C E Carty K J Hofmann P M Keller M A Polokoff R J Lynch B J Keech R J Gould R Z Maigetter L D Schultz |
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| Institution: | (1) Department of Cellular and Molecular Biology, Merck Sharp and Dohme Research Laboratories, 19486 West Point, PA;(2) Department of Biological Chemistry, Merck Sharp and Dohme Research Laboratories, 19486 West Point, PA |
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| Abstract: | Summary The  -mating factor pre-pro-leader sequence under the regulation of theGAL10 promoter was used to direct the secretion of echistatin by recombinant yeasts. Optimization of the culture medium and host strain increased the productivity of shake flask cultures twenty-fold to 8 mg/L. In fermentors, the production of echistatin was greater than 40 mg/L. |
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