Ovarian tissue features assessed in bovine fetuses after vitrification and xenotransplantation procedures |
| |
| Institution: | 1. Laboratory of Animal Reproduction, Federal University of Uberlândia, Uberlândia, MG, Brazil;2. Cytogenetic Laboratory, Genetic and Biochemistry Institute, Federal University of Uberlândia, Uberlândia, MG, Brazil;3. Conception Biology, Berkeley, 94710, CA, USA;1. Department of Obstetrics, Jingmen First People''s Hospital, Jingmen City, 448000, Hubei Province, China;2. Department of Gynecology, Jingmen First People''s Hospital, Jingmen City, 448000, Hubei Province, China;1. Department of Obstetrics, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China;2. Department of Biochemistry and Molecular Biology, GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, China;1. Department of Thyroid and Breast Surgery, the Affiliated Peoples Hospital of Ningbo University, Ningbo City, Zhejiang Province, 315040, China;2. Department of Nail Breast Surgery, Huai''an Second People''s Hospital, Huai''an City, Jiangsu Province, 223002, China;1. CNRS, IFCE, INRAE, Université de Tours, PRC, 37380, Nouzilly, France;2. Union Evolution, Noyal-Sur-Vilaine, France;3. INRAE, Université de Tours, CHU de Tours, PIXANIM, 37380 Nouzilly, France;4. INRAE, Université de Tours, ISP, 37380 Nouzilly, France;5. Tours University, Tours, France;1. Department of Obstetrics and Gynaecology, Faculty of Medicine, Albert Szent-Györgyi Medical and Pharmaceutical Centre, University of Szeged, Szeged, Hungary;2. Department of Laboratory Medicine, Faculty of Medicine, Albert Szent-Györgyi Medical and Pharmaceutical Centre, University of Szeged, Szeged, Hungary;3. Department of Obstetrics and Gynaecology, Danderyd Hospital, Stockholm, Sweden |
| |
| Abstract: | Cryopreservation and transplantation of ovarian tissue are proposed methods for the restoration of endocrine function and reproductive potential. Therefore, this study aimed to evaluate the effects of vitrification and xenotransplantation on follicle viability, activation, stromal cell integrity, vascularization, and micronuclei formation. Bovine fetal ovaries were fragmented and assigned to the following groups: Fresh control (FC), ovarian fragments immediately fixed; Vitrified control (VC), ovarian fragments vitrified; Vitrified xenotransplanted (VX), ovarian fragments vitrified and xenotransplanted; and Fresh xenotransplanted (FX), ovarian fragments xenotransplanted. Ovarian fragments were grafted in female BALB/c mice and recovered after 14 days. Follicular viability was preserved (P > 0.05) in VC group. The rate of developing follicles was greater (P < 0.05) in the FX group compared to other groups. Follicular density was higher (P < 0.05) in the VC group than the FC, VX, and FX groups. A decrease (P < 0.05) of stromal cell density was recorded after vitrification (VC vs. FX). Blood vessel density decreased in VC, VX, and FX groups compared with the FC group, and blood vessel density was correlated with follicular viability (positively; P = 0.07) and developing follicles (negatively; P < 0.001). Both vitrification and xenotransplantation groups (VC, VX, and FX) had a greater (P < 0.05) number of cells with one MN compared to the FC group. In summary, our findings showed that both vitrification and xenotransplantation modified blood vessel, follicular and stromal cell densities, follicular viability and activation, and micronuclei formation in ovarian tissue. |
| |
| Keywords: | Chromosomal abnormalities Preantral follicles Stromal cells Vascularization Vitrification |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
