Long Non-coding RNA HOTAIR Is Targeted and Regulated by miR-141 in Human Cancer Cells |
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| Authors: | Takeshi Chiyomaru Shinichiro Fukuhara Sharanjot Saini Shahana Majid Guoren Deng Varahram Shahryari Inik Chang Yuichiro Tanaka Hideki Enokida Masayuki Nakagawa Rajvir Dahiya Soichiro Yamamura |
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| Affiliation: | From the ‡Department of Urology, San Francisco Veterans Affairs Medical Center and University of California, San Francisco, San Francisco, California 94121.;the §Department of Oral Biology, College of Dentistry, Yonsei University, Seoul 120-752, Korea, and ;the ¶Department of Urology, Graduate School of Medical and Dental Sciences, Kagoshima University, Kagoshima 890-8520, Japan |
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| Abstract: | ![]()
HOTAIR is a long non-coding RNA that interacts with the polycomb repressive complex and suppresses its target genes. HOTAIR has also been demonstrated to promote malignancy. MicroRNA-141 (miR-141) has been reported to play a role in the epithelial to mesenchymal transition process, and the expression of miR-141 is inversely correlated with tumorigenicity and invasiveness in several human cancers. We found that HOTAIR expression is inversely correlated to miR-141 expression in renal carcinoma cells. HOTAIR promotes malignancy, including proliferation and invasion, whereas miR-141 suppresses malignancy in human cancer cells. miR-141 binds to HOTAIR in a sequence-specific manner and suppresses HOTAIR expression and functions, including proliferation and invasion. Both HOTAIR and miR-141 were associated with the immunoprecipitated Ago2 (Argonaute2) complex, and the Ago2 complex cleaved HOTAIR in the presence of miR-141. These results demonstrate that HOTAIR is suppressed by miR-141 in an Ago2-dependent manner. |
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| Keywords: | Cancer Biology Cell Growth Gene Regulation Invasion MicroRNA Genistein HOTAIR Long Non-coding RNA miR-141 Renal Carcinoma |
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