Bio-modulated mice epithelial endometrial organoids by low-level laser therapy serves as an invitro model for endometrial regeneration |
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| Institution: | 1. Department of Reproductive Health and Family Planning, National Research Centre, 33th El Buhouth St., Dokki, Giza, 12622, Egypt;2. Department of Animal Reproduction and Artificial Insemination, National Research Centre, 33th El Buhouth St., Dokki, Giza, 12622, Egypt;3. Department of Medical Application of Laser, National Institute of Laser Enhanced Sciences, Cairo University, 1 Gamaa Street, Giza, 12613, Egypt;1. Centre for Human Reproductive Science (ChRS), Institute of Metabolism and Systems Research, College of Medical & Dental Sciences, University of Birmingham, United Kingdom;2. Birmingham Women’s Fertility Centre, Birmingham Women’s Hospital, Birmingham Women’s and Children’s Hospital NHS Foundation Trust, Birmingham, United Kingdom;3. School of Biosciences, University of Birmingham, United Kingdom;1. Department of Obstetrics, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, Beijing, China;2. Department of Biochemistry and Molecular Biology, GMU-GIBH Joint School of Life Sciences, Guangzhou Medical University, Guangzhou, China;1. Department of Obstetrics, Jingmen First People''s Hospital, Jingmen City, 448000, Hubei Province, China;2. Department of Gynecology, Jingmen First People''s Hospital, Jingmen City, 448000, Hubei Province, China;1. Laboratory of Animal Reproduction, Federal University of Uberlândia, Uberlândia, MG, Brazil;2. Cytogenetic Laboratory, Genetic and Biochemistry Institute, Federal University of Uberlândia, Uberlândia, MG, Brazil;3. Conception Biology, Berkeley, 94710, CA, USA;1. Department of Obstetrics, The Maternity Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, 530021, China;2. Center for Genomic and Personalized Medicine, Guangxi Medical University, Nanning, Guangxi, 530021, China;3. Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, 200092, China;4. The First Affiliated Hospital of Guangxi Medical University, Nanning, Guangxi, 530021, China;5. Guangxi Key Laboratory of Genomic and Personalized Medicine, Nanning, Guangxi, 530021, China;6. Guangxi Collaborative Innovation Center for Genomic and Personalized Medicine, Nanning, Guangxi, 530021, China |
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| Abstract: | Endometrial regeneration is a dynamic process that is not well understood. The destruction of the endometrium with the formation of intrauterine adhesions is known as Asherman’s syndrome. The lesions range from minor to severe adhesions and their impact on pregnancy is well documented. Operative hysteroscopy is the mainstay of diagnosis and treatment of intrauterine adhesions. Nevertheless, the recurrence rates remain high. It was recorded that low-level laser therapy in low doses has a stimulatory effect on different tissues while the high dose produces a suppressive effect. Organoid is a three-dimensional assembly that displays architectures and functionalities similar to in vivo organs that are being developed from human or animal stem cells or organ-specific progenitors through a self-organization process. Our prospective was to study the effect of Low-Level Laser Therapy (LLLT) on mouse epithelial endometrial organoids regarding cell proliferation and endometrial regeneration as a new modality of treatment. An in vitro clinical trial to generate mouse epithelial organoid model and testing LLLT using He:Ne 632.8 nm device on organoids proliferation, function, and their response to ovarian hormones was performed. Trying endometrial regeneration by culturing organoids with decellularized uterine matrix (DUM) and studying the LLLT effect on the regeneration process. LLLT produced a proliferative effect on the epithelial mouse organoids confirmed by Ki67 and PCNA IHC. The organoids could regenerate the epithelial layer of the endometrium in vitro on DUM and LLLT could help in this process. In conclusion, organoids whether control or bio-stimulated proved a new modality to regenerate the endometrium. |
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| Keywords: | LLLT Organoids Asherman’s syndrome Endometrial regeneration |
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