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Influence of Cr Adhesion Layer on Detection of Amyloid-Derived Diffusible Ligands Based on Localized Surface Plasmon Resonance
Authors:Shaoli Zhu  ChunLei Du  Yongqi Fu  Qiling Deng  Lifang Shi
Affiliation:(1) State Key Laboratory of Optical Technologies for Microfabrication, Institute of Optics and Electronics, Chinese Academy of Sciences, Chengdu, 610209, People’s Republic of China;(2) School of Physical Electronics, University of Electronic Science and Technology of China, Chengdu, 610054, Sichuan Province, People’s Republic of China
Abstract:A Cr adhesion layer inserted between Ag nanoparticles and a glass substrate, for the purpose of improving the adhesion of Ag nanoparticles to glass, was observed to cause an abnormal peak shift of extinction spectra in non-specific reactions. The undesired peak shift misleads molecule detection in non-specific reactions. To solve this issue, a practical technique using n-propyl-trimethoxysilane-based passivation for the detection of amyloid-derived diffusible ligands was investigated as a route to eliminate the abnormal peak shifting observed in the non-specific reactions. To evaluate this passivation technique, localized surface plasmon resonance immunoassay experiments were conducted. Experimental results derived with and without the passivation process were investigated as a basis for comparative analysis. Our experimental results demonstrate that this passivation technique effectively eliminates the observed peak shift originating from the Cr adhesion layer.
Keywords:LSPR  ADDLs  Anti-ADDL  Passivation  Cr adhesion layer
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