Aseptic cultivation of higher plants

Summary A short survey of different problems connected with the aseptic cultivation of higher plants is given.Accounts have been given of experiments in which antibiotics and fungicides have been used, as well separately as in combination, as surface-sterilizing agents.The use of chlorine as a sterilizing agent for the surface-sterilization of pea seed has been investigated and an account given of the importance of damage to the skin of the seed in the procedure.Procedures for the surface sterilization of oats and barley, and for pea seed, have been developed.Various arrangements in which the aseptic cultivation of higher plants may be carried out are described.