Molecular characterization of <Emphasis Type="Italic">Vigna radiata</Emphasis> (L.) Wilczek genotypes based on nuclear ribosomal DNA and RAPD polymorphism |
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Authors: | Aparna Raturi S K Singh Vinay Sharma Rakesh Pathak |
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Institution: | (1) Central Arid Zone Research Institute, Jodhpur, Rajasthan, India;(2) Banasthali Vidyapith, Tonk, 304022, Rajasthan, India; |
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Abstract: | Mungbean germplasm characterization, evaluation and improvement are fundamentally based on morpho-agronomic traits. The lack
of break-through in mungbean production has been due to non-availability of genetic variability for high yield potential.
Forty-four genotypes of mungbean Vigna radiata (L.)Wilczek] were subjected to random amplified polymorphic DNA (RAPD) analysis to assess the genetic diversity and relationships
among the genotypes. Multilocus genotyping by twelve RAPD primers generated 166 markers and detected an average of intraspecific
variation amounting to 82% polymorphism in banding patterns. Dendrogram obtained from cluster analysis delineated all the
44 genotypes into six clusters. Higher values of Nei’s gene diversity (h) and Shannon information index (i) and genetic distance analysis validate existence of wide genetic diversity among mungbean genotypes tested. Besides internal
transcribed spacer (ITS) length variations, single nucleotide polymorphisms (SNPs) and insertions/deletions (INDELS) were
detected at number of sites in nuclear rDNA region and the sequences of representatives of each sub-cluster and all distinct
genotypes have been submitted to NCBI database and assigned Gen accession numbers HQ 148136-148147. Multiple sequence alignment
revealed further lineages of distinct genotypes with main RAPD clusters. The measures of relative genetic distances among
the genotypes of mungbean did not completely correlate the geographical places of their development. The homogeneous phenotypic
markers proved insufficient in exhibiting genetic divergence among mungbean genotypes studied. RMG-62, RMG-976, and NDM-56
have been identified as potential source of parents for crop improvement. RAPD primers, OPA-9 and OPA-2 as polymorphic genetic
markers and number of pods/plant and number of seeds/plant as dependable phenotypic markers have been identified for improving
yield potentials. This genetic diversity will be of significance in developing intraspecific crosses in mungbean crop improvement
programme. |
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