| 应用AR—CM—MIC阳离子测定系统检测单个神经元内游离钙 |
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| 引用本文： | 张维宁 温和瑞. 应用AR—CM—MIC阳离子测定系统检测单个神经元内游离钙[J]. 中国应用生理学杂志, 1993, 9(4): 344-348 |
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| 作者姓名： | 张维宁 温和瑞 |
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| 作者单位： | 南京大学生物系，南京大学生物系，南京大学生物系，南京大学配位化学国家重点实验室，南京大学配位化学国家重点实验室，南京大学配位化学国家重点实验室，南京大学配位化学国家重点实验室 南京 210008，南京 210008，南京 210008，南京 210008 |
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| 摘 要： | 运用Ca~(2 )指示剂Fura-2作为细胞内钙离子的荧光探针,采用精密的AR-CM-MIC阳离子测定系统,检测了分离的单个神经细胞内游离钙离子浓度的动态变化,同时观察了钙离子载体、钙螯合剂等多种药物对细胞内钙浓度的影响,并追踪刺激前后的瞬间变化,探讨此项技术应用于检测细胞内游离钙的灵敏度及适用范围,取得了良好的效果。
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| 关 键 词： | Fura-2/AM 脑细胞 钙 阳离子测定系统 |
DETERMINATION OF INTRACELLULAR FREE CALCIUM IN DISSOCIATED BRAIN CELLS USING CATION MEASUREMENT SYSTEM AR-CM-MIC |
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| Zhang Weining,Wu Fumei,Zhang Zuxuan,Wen Herui,Ling JingWang Jinxi,Chen Rongsan. DETERMINATION OF INTRACELLULAR FREE CALCIUM IN DISSOCIATED BRAIN CELLS USING CATION MEASUREMENT SYSTEM AR-CM-MIC[J]. Chinese journal of applied physiology, 1993, 9(4): 344-348 |
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| Authors: | Zhang Weining Wu Fumei Zhang Zuxuan Wen Herui Ling JingWang Jinxi Chen Rongsan |
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| Abstract: | Intracellular free Ca2 concentration was measured with Fura-2 in freshly dissociated brain cells prepared from newborn ( 24h ) mice using AR-CM-MIC cation measurement system. The temporal changes of intracellular calcium ion concentrations after treatment with Ca2 ionophore, protein synthesis inhibitor anisomycin and so on were also observed. The results showed that the system for monitoring intracellular Ca2 concentration dynamics with Fura-2 was rapid, efficient and sensitive. |
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| Keywords: | Fura-2 / AM Brain cells: Ca2 cation measurement system |
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