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重组白细胞介素2制品外源残余DNA的检测
引用本文:金立杰,张淑子.重组白细胞介素2制品外源残余DNA的检测[J].微生物学免疫学进展,1996,24(3):28-29.
作者姓名:金立杰  张淑子
作者单位:卫生部长春生物制品研究所
摘    要:采用DNA杂交技术,用32P标记的探针检测重组白细胞介素2制品的外源DNA残余量。设三个水平:25个剂量、15个剂量、5个剂量。结果提示该方法是一种敏感有效的检测方法,其最小用样量为5个剂量单位。这为新制品检测方法的建立提供依据。

关 键 词:重组白细胞介素2,外源残余DNA,检测

Detection of Exogenous Residual DAN in Recombinant Interleukin 2 Products
Jin Lijie,Zhang Shuzi,Zhang Shian.Detection of Exogenous Residual DAN in Recombinant Interleukin 2 Products[J].Progress In Microbiology and Immunology,1996,24(3):28-29.
Authors:Jin Lijie  Zhang Shuzi  Zhang Shian
Abstract:The technique of DNA hybridization was applied to detect exogenous residual DNA in rIL-2 productrs with 32 P as a probe. There were three levels:25 doses, 15 doses and 5 doses, in this test. The result showed the method was a sensitive and effective way, and the smallest doses of sample was 5 doses.It provides a basis for establishing method to detect a kind of new product
Keywords:Recombinant  Interleukin  2    Exogenous  residual  DNA    Detection
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